Topics included are DNA and RNA structure, restriction enzymes, growth of bacteria, replication, transcription and translation. Using a pair of priming complementary sequences (oligonucleotide primers) flanking a location of interest, together with unique heat-resistant polymerases (DNA copying enzymes), multiple copies of a targeted chimeric gene can be obtained (Figure (Figure2 2 ) . In particular, molecular biology seeks to understand the three-dimensional structure of biological macromolecules through techniques such as X-ray diffraction and electron microscopy . Cell Biology: Technique # 9. History of Molecular Biology. Hands-on Basic Molecular Biology Techniques Share This is a 5-day training workshop that will enable participants acquire the basic molecular techniques required for success in scientific research. Despite its prominence in the contemporary life sciences, molecular biology is a relatively young discipline, originating in the 1930s and 1940s, and becoming institutionalized in the 1950s and 1960s. Various techniques have been developed for molecular biology, though researchers in the field may also employ methods and techniques native to genetics and other closely associated fields. ADVERTISEMENTS: Some of the most important techniques used in molecular biology are as follows: Molecular Biology techniques include characterization, isolation and manipulation of the molecular components of cells and organisms. Students will learn hands on laboratory techniques found in most molecular biology labs. Spectroscopy: Variety of techniques are developed with the principle of spectroscopy, i.e., study of interac­tion between electromagnetic radiation and the substance. 8.1: Isolating Genomic DNA DNA purification strategies rely on the chemical properties of DNA that distinguish it from other molecules in the cell, namely that it is a very long, negatively charged molecule. 1. PCR is the most frequently used molecular technique in a molecular pathology laboratory. have electromagnetic waves with average speed of 3 x 10 8 m/sec. Light, heat, microwaves, infra-red, X-rays etc. Usually, molecular genetics experiments involve some combination of techniques to isolate and analyze the DNA or RNA transcribed from a particular gene. This course is a combination of lectures and hands-on laboratories in Molecular Biology and recombinant DNA techniques.